Introduction

Components	Shipping Condition	Unit Size	Product Line	Detection Method
E. coli DNA Control	-20℃	100 Reactions	SHENTEK™	Probe-based qPCR
qPCR Master MIX
DNA Dilution Buffer (DDB)
E. coli Primer&Probe MIX (Incl IPC)	-20℃, protect from light

Overview

SHENTEK™ Residual E. coli DNA Quantitation Kit is used to quantitate residual E. coli host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to perform rapid, specific, and reliable quantitation assay at the fg level. IPC(Internal Positive Control) is included in the E. coli Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK™ Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

Specification

Range	3x10-2-3x102 pg/μL, R2=0.99968, AE=97.5%
Accuracy	Recovery = 81.2%-107.4% , CV<30%
LOQ	0.03 pg/μL
LOD (LLOQ)	0.003 pg/μL
Precision	2.2%-17.9% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, HZSKBio qPCR equipments

Related Products

SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

Documents

User Guide

Residual E. coli DNA Quantitation Kit (2G)-SHENTEK™